| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
|
| País: |
Chile
|
| Título: |
Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene
|
| Autores: |
Zhang,Yue
Xia,Han
Yuan,Mei
Zhao,Chuanzhi
Li,Aiqin
Wang,Xingjun
|
| Data: |
2012-01-01
|
| Ano: |
2012
|
| Palavras-chave: |
Arachis hypogaea L.
CDNA library
Chalcone isomerase
Expression analysis
|
| Resumo: |
Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than the expression level in stem and leaf. AhCHI was expressed in a high level in seeds with a purple seed coat, while its expression was low in seed with white seed coat.
|
| Tipo: |
Journal article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100005
|
| Editor: |
Pontificia Universidad Católica de Valparaíso
|
| Formato: |
text/html
|
| Fonte: |
Electronic Journal of Biotechnology v.15 n.1 2012
|
